Flash frozen tissues are non-fixed post-surgical tissue samples frozen in liquid nitrogen (LN2). This method allows great structural and biochemical preservation of the tissue and is the format of choice for a majority of downstream analysis.
All samples are collected under IRB approval and are prepared in strict accordance with our established processing protocol. Quality control by a certified pathologist is performed on all samples.
Flash Frozen Tissue Formats
Unless specifically indicated, standard frozen tissue samples weigh between 0.5-1.0 grams. Frozen cancer tissues have the percent of tumor nuclei and tumor necrosis content verified by a pathologist.
Tissues can be frozen by submerging in LN2 or in Optimal Cutting Temperature (OCT) media. Flash frozen samples are stored at temperatures between -140°C and -180°C in the vapor-phase of LN2.